The Science Behind Vioni+ — VyVa

Vioni+ Ionic Vitality — The Science

The research behind Ioniplex®

A patented fulvic ionic mineral complex with 65+ trace minerals. Two issued US patents. One randomized, double-blind, placebo-controlled clinical evaluation on nail, hair and skin condition. Multiple in vitro investigations on absorption, collagen pathways, glycation and mitochondrial metabolism.

How to read this page. Most Ioniplex® research consists of manufacturer-conducted laboratory studies (Sunny Biodiscovery, Santa Paula, California) and case studies — not independent peer-reviewed human randomized trials. We label every source by tier so you know what you're looking at. We never present manufacturer data as "clinical trial" data.

Independent Clinical Study

Tier B2 — Manufacturer-sponsored clinical evaluation Randomized, double-blind, placebo-controlled6 weeks

Boisits (2009) — Six-Week Evaluation of Oral Ioniplex on Nail Condition

Investigator: Edward K. Boisits, PhD. Study Coordinator: Rosemary McNicol, B.S., CCRC. Site: International Research Services, Inc., Port Chester, NY. Sponsor: Mineral BioSciences, LLC. Study No. 3411BK0609.

A randomized, double-blind, placebo-controlled clinical evaluation of two oral Ioniplex formulations against a control, in three groups of healthy female subjects, over six weeks. Nail growth was measured instrumentally using digital photography and image analysis; nail, hair and skin condition were assessed by an expert evaluator and by the panellists themselves.

Nail growth ran 142% and 141% of baseline in the two Ioniplex groups, against 129% in the control group. Skin texture improved 33% from baseline at six weeks. Hair condition improved 14% overall. Nail strength and hair thickness both increased significantly at week six in the higher-dose group, and hair luster changed significantly in the second. (Full clinical report on file with Mineral BioSciences.)

Why this matters: This is the closest thing in the Ioniplex research portfolio to an independent clinical evaluation — randomized, double-blind, placebo-controlled — even though it was sponsored by the manufacturer and has not been published in a peer-reviewed journal. That is exactly why we send you the report rather than quoting a headline from it.

Manufacturer Laboratory Research — Mechanism & Bioavailability

Tier C — Manufacturer in vitroSunny Biodiscovery Report 697-BIOAV2015

Bioavailability of Ioniplex™ in Human Cells (in vitro)

Lab: Sunny Biodiscovery, Inc. (Santa Paula, CA). Prepared for Mineral BioSciences, March 23, 2015.

When human cells were incubated with Ioniplex and then rinsed, the fluorescence signal in treated cultures was over ten times higher than in controls — indicating that Ioniplex associates with cells and cell membranes. Quantification of a magnesium-binding dye showed an overall increase in intracellular magnesium in treated cell populations.

Why this matters: Vioni+'s premise is that mineral nutrition is about delivery, not dose. A mineral you cannot absorb is a mineral you did not take. This in vitro report supports that mechanism story at the cellular level. It is not, however, a human bioavailability trial — and we're explicit about that.

Tier C — Manufacturer in vitroSunny Biodiscovery Report 1284PAMPA2021

Epithelial Permeation of Turmeric With and Without Ioniplex (PAMPA model)

Lab: Sunny Biodiscovery, Inc. (Santa Paula, CA), May 11, 2021. Prepared for Mineral BioSciences. Millipore PAMPA membrane model — the standard laboratory stand-in for passive intestinal absorption. Turmeric extract tested alone and in a 1:1 combination with Ioniplex, in triplicate.

Turmeric permeation rose from an average of 8.1% to 9.5% at three hours — a 17.5% increase, p = 0.026 — and from 9.1% to 10.5% at five hours, a 15.9% increase, p = 0.003. The report's conclusion: a statistically significant increase, "demonstrating its potential to enhance the passive intestinal absorption of nutrients, in vivo."

Why this matters: Turmeric is among the most-bought and least-absorbed supplements on the shelf — which is precisely why the category sells piperine, phytosome and oil-carrier versions at a premium. If you already take turmeric, you are already paying for absorption. This is a membrane model rather than a human absorption study, and we say so plainly; but it is the clearest illustration of what a fulvic ionic carrier is actually for, and it is why Vioni+ is a morning dose taken alongside the rest of your routine.

Tier C — Manufacturer in vitroSunny Biodiscovery2006 / rev. 2014

Effect of Ioniplex™ on Mitochondrial Metabolism in Cell Culture

Lab: Sunny Biodiscovery, Inc. Comparative MTT assay (a standard measure of mitochondrial respiratory activity) on human cells. Ioniplex™ compared against two leading commercial energy drinks (No Fear, Full Throttle).

Among the materials tested, Ioniplex™ produced the greatest stimulation of mitochondrial metabolic activity — over 50% at certain concentrations — and was above or on par with both energy drinks. In a separate DNA microarray study measuring the expression of more than 20,000 genes, Ioniplex stimulated a range of genes involved in mitochondrial metabolism, respiration and ATP production.

Why this matters: The mechanistic basis for Ioniplex's cellular energy positioning. Cell-culture data, not human data — but consistent across two independent read-outs.

Tier C — Manufacturer in vitroSunny Biodiscovery Report 400COL2010

Effect on Type I Collagen Levels in Human Dermal Fibroblasts (in vitro)

Lab: Sunny Biodiscovery, Inc. April 12, 2010. Prepared for Global Organics.

In vitro assay testing the effect of Ioniplex-containing test materials on Type I collagen secretion by aged dermal fibroblast cells.

Ioniplex repeatedly stimulated Type I collagen in the fibroblast cells — averaging approximately 30%, and as much as quadruple the water control depending on the cell line used.

Why this matters: Collagen production is a key concern for skin appearance after 35. This cell-culture data describes one pathway by which the trace minerals in Ioniplex may support skin's natural processes. Cell-culture results don't always translate to humans — we're cautious about overclaiming.

Tier C — Manufacturer in vitroReports MX3 & AD0706182007

Effect on Advanced Glycation End-products (in vitro)

Labs: Sunny Biodiscovery, Inc. and BIOalternatives. Two separate assays measuring non-enzymatic glycation against a water control, with aminoguanidine — a known inhibitor of glycation — as the positive reference.

Various batches of Ioniplex produced up to a 50% decrease in advanced glycation end-products, dose-dependently, and comparable with aminoguanidine. A second laboratory, working independently, reproduced the direction of the result across all doses tested.

Why this matters: This is the basis on which Ioniplex was patented as an anti-glycation agent (US 8,927,031, listed below). It is cell-free and cell-culture data describing a chemical process — not a human outcome, and we do not present it as one.

Tier C — Manufacturer in vitroSunny Biodiscovery Report 466A2011

Commercial Formulations With vs Without Ioniplex on Muscle and Bone Cells

Lab: Sunny Biodiscovery, Inc. February 22, 2011. Prepared for Mineral BioSciences.

Compared Isagenix and Ensure (commercial supplement beverages) with and without added Ioniplex on human skeletal muscle (HSMC) and bone cell populations.

Adding Ioniplex to commercial formulations was associated with increased metabolic activity and protein production in muscle and bone cell models.

Why this matters: Supports the broader story that Ioniplex's trace mineral nutrition may be relevant beyond skin and nail health. Pre-clinical data only — but a useful frame for the "cellular nutrition" positioning.

Manufacturer Technical Dossier

Tier C — Manufacturer white paper2020 / 2021

Ioniplex White Paper

Publisher: Mineral BioSciences, Goodyear, Arizona. Comprehensive technical dossier covering Ioniplex's composition (over 65 major, minor and trace minerals in ionic form, with the guaranteed composition published in full), sourcing from mineraloid veins across North America, the patented extraction process, applications, and the supporting research bibliography.

How we use it: Background reference and footnote source. Specific claim percentages from this white paper are always qualified as manufacturer laboratory data on file — never as "clinical research" or "proven."

Patents

US Patent 8,709,497Granted

Mineral, Cosmetic, Pharmaceutical, and Agriculture Compositions and Methods for Producing the Same

Mineral BioSciences®. Covers methods for producing mineral compositions including a large number of naturally occurring minerals that facilitate delivery into the body — for cosmetic, pharmaceutical, agricultural, and nutraceutical applications.

US Patent 8,927,031Granted

Anti-Glycation Methods and Compositions

Mineral BioSciences®. Covers compositions used to address glycation events in humans and animals.

US Patent PendingFiled 2016

Methods and Compositions for Modulating Muscle and Bone Loss

Mineral BioSciences®. Pending application. Methods to address age-related muscle degeneration in humans and animals.

Manufacturing & Quality Assurance

FDA Reg. No. 10063446198cGMP-compliant

Manufactured in an FDA-registered facility

Vioni+ is manufactured in an FDA-registered, cGMP-compliant facility.

ISO-accredited testingBatch WG-251206

Independent batch testing — FARE Labs Private Limited

Current production batch verified:

Ioniplex per gummy: 208.14 mg (label spec NLT 90% of declared)
Heavy metals (As, Pb, Hg, Cd): all below limit of quantification
Pathogens: all absent
Total aerobic count: <10 cfu/g (spec NMT 1,000)
Sugar: not detected

Required disclaimers. These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. Individual results may vary. Not intended for pregnant or breastfeeding women or children under 18. Manufactured in an FDA-registered, cGMP-compliant facility. Independently tested by an ISO-accredited laboratory. Ioniplex® is a registered trademark of Mineral BioSciences®, US Patents 8,709,497 and 8,927,031 + 1 patent pending.